The Michaelis–Menten equation is an expression of the relationship between the initial velocity V 0 of an enzymatic reaction and substrate concentration [ S ] . There are three conditions that are useful for simplifying the Michaelis–Menten equation to an expression from which the effect of [ S ] on the rate can be more readily determined. Match the condition (e. g., [ S ] = K m ) with the statement or statements that describe it.1. Doubling [S] will almost double the rate. 2. Half of the active sites are occupied by substrate. 3. About 90% of the active sites are occupied by substrate. 4. Doubling [S] will have little effect on the rate. 5. Less than 10% of the active sites are occupied by substrate. 6. This condition will result in the highest rate.