Chemistry, 21.04.2020 18:35 jasminecoronetti44

Ampicillin is a bacteriostatic antibiotic. (Instead of killing bacteria, it inhibits their growth.) Ramon plated bacteria transformed with a plasmid that confers ampicillin resistance. He plated the bacteria on Thursday and left them in a 37 oC incubator overnight. On Friday, he observed moderately sized bacterial colonies. He decide to leave them in the incubator a little longer before picking the colonies that he wanted to work with further. Unfortunately, he forgot about the plate and left it in the incubator over the weekend. On Monday, his plate had large colonies that were each surrounded by very small colonies

A) Interpret and explain Ramon’s observations.

B) Predict what he will find when he picks some large and some small colonies and follows the plasmid isolation protocol for each of the colonies.

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Ampicillin is a bacteriostatic antibiotic. (Instead of killing bacteria, it inhibits their growth.)...

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