BASIC CALCULATIONS IN SPECTROSCOPY 1. Calculate the %T of light at 425nm if the light entering the cell is 200 lumens and the amount of light exiting the cell is 50 lumens.
2. Calculate the absorbance of the above problem.

25%

Explanation:  

1. Transmittance, T= P/P0  

Where P = light exiting the cell

           P0 = light entering the cell

Therefore %T =  P/P0 ×100

           = 50/200×100

=25%

2. Absorbance, A= -log(T)

But T= 0.25

Therefore A= -log(0.25)

                  = 0.6020

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