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Chemistry, 10.06.2020 16:57 bvaughn4152

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master mix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps. Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest. Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest. dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template

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When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, t...
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