if you are using a formula where you need the change in temperature, explain why it is not important whether your temperatures are both in Kelvin or Celsius?

Imagine that you have produced several versions of lactase, each of which differs from normal lactase by a single amino acid. describe a test that could indirectly determine which of the versions significantly alters the three-dimensional shape of the lactase protein.

Straightforward questions answered in the powerpoint slidesreaction: heating the starting materials under refluxwhat does it mean to heat under reflux? why do we choose water as the reflux solvent? what are boiling chips used for? why do we put a condenser on top of the reaction? why do we add heat and let the reaction stir for 30 minutes? why do we add sulfuric acid to the reaction after it cools as opposed to when it’s still hot? separation: filtration of precipitatewhy don’t we do an aqueous and organic extraction in the separatory funnel? why do you rinse the salicylic acid on the filter with ice cold water? purification: recrystallization of salicylic acid (no hot filtration needed)what is the difference in the amount of room temperature water vs. boiling water needed to dissolve the salicylic acid (assume a 1.2 gram yield of salicylic acid)? remember, in the lab if you need x ml of boiling water to dissolve a solid, then you should add a little more (definitely no more than 1.5 times the theoretical amount) to ensure it doesn’t recrystallize prematurely.analysis: melting point of salicylic acidwhat can you conclude if the melting point of the salicylic acid you just synthesized is 152-155oc and the 1: 1 mix of your product and “synthetic” salicylic acid is 151-154oc?

15. which of the following is not human-caused groundwater pollution? a. water in an aquifer dissolves elements such as arsenic and mercury from surrounding rock. b. water in an aquifer is contaminated by leachate that seeps into the ground from a landfill. c. water in an aquifer becomes polluted with chemicals used in hydraulic fracturing, or fracking. d. water in an aquifer absorbs harmful bacteria from the drainage field of a septic tank.