Weigh out the appropriate amount of HEPES sodium salt required to prepare 100 mL of a HEPES buffer that has pH 7.50 and is 90 mM in HEPES. FW for the HEPES sodium salt is 260.31. Transfer to a 150 mL beaker. Flowchart: Calculate the amount of HEPES sodium salt required to make the buffer.

2.343 g of HEPES sodium salt is needed.

Explanation:

So, from the question above we have the following parameters which is going to help in solving this particular question.

The number of moles = 90mM, the pH = 7.50, FW for the HEPES sodium salt = 260.31, and the volume of HEPES sodium salt required = 100mL.

Therefore, the amount of HEPES sodium salt required to make the buffer = 260.31 × 9 × 0.001 = 2.343 grams of HEPES sodium salt is needed in 10mL.

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