The concentration of yeast t-RNA in an aqueous solution is found to be 10 µg/mL Upon testing in the spectroscope the absorbance is was found to be 0.209 and when this solution was placed in a 1.00 cm cuvette and a certain monochromatic radiation of 258 nm radiation was passed through it. Answer the following questions. a. Calculate the specific absorptivity (extinction coefficient), including units, of yeast t-RNA.
b. What will be the absorbance if the solution is diluted to 5 μg/mL?
c. What will be the absorbance if the path length of the original solution is increased to 5.00 cm?