How does the spectrophotometer measure absorbance? Why does the dye absorb light (electron transitions)? What color light does Blue #1 dye absorb? What is the lambda max of the dye? What is Beer’s law and how can it be used to calculate the desired concentrations for your solutions? Considering Beer’s law, is it possible to look up the molar absorptivity coefficient of Blue #1 dye? How will you estimate what concentrations of dye will be required for an absorbance of 1.0 AU given a path length of 1.46cm? How would you dilute a 2.0 mM dye solution to make 100mL of this 1.0 AU solution? How can the 1.0 AU solution be diluted to form a point on the calibration curve at 0.25 AU?

(04.05 hc) analyze the given diagram of the carbon cycle below. an image of carbon cycle is shown. the sun, a cloud, two trees, one on the left and the other on the right, an animal, lake, and a factory are shown in the image. an arrow is shown from the sun towards the left tree marked a. the sun is marked b. there is an arrow from the air above the clouds, marked c, towards the left tree. an arrow from a location close to the ground marked d points towards dead organisms, which is a label under the animal. an arrow marked e points from the right tree straight up to the clouds. an arrow marked f points from the animal straight up to the clouds. an arrow marked g points from the factory towards the air above the clouds, c. there is an arrow pointing from the air to the lake labeled carbonates in water, an arrow pointing down from dead organisms to fossils and fossil fuels, and an arrow from fossils to the factory. part 1: which compound does c represent? part 2: name a process that could release this compound into the air. part 3: explain how the elements that form it are conserved during the carbon cycle. use complete sentences to explain your answer.