The fluorescence of a CFP solution is quenched with a 30 mM solution of YFP, where the observed fluorescent lifetime is 0.5 s and the quenching rate is 43.0 M-1 s-1. Use the Stern-Volmer equation to find the ratio of non-quenched to quenched fluorescence, and then use the inverse of this ratio to calculate the percentage change in fluorescence upon quenching.
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The fluorescence of a CFP solution is quenched with a 30 mM solution of YFP, where the observed fluo...
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