1a - Design primers to clone your gene Phenylalanine hydroxylase into pET28a plasmid. 1b -. Explain your reason behind designing the specific primers: Restriction sites, presence of tags.

when [oh-] is 10-5  m, and [h3o+] is 10-9  m, the poh is 5 and ph is 9. again in both cases the sum of ph and poh is 14. however, the hydrogen ion concentration is not always going to be equal to exactly 1 x 10 raised to a negative number. for example, we skipped over the value of 2.0 x 10-7.

plants need sunlight, water, and carbon dioxide to make food. plants use the raw materials of water, nutrients they absorb from soil, and carbon dioxide to make sugars the plant can use. energy from the sun and chlorophyll are also needed.

the positions which points up in upward carbon and down in downward carbon are axial

and vice versa holds true for equatorial positions

so the correct answer with labelled diagram will be

aquifers are bounded above and below by impermeable rock. the driving force of groundwater movement in confined aquifers is pressure, rather than gravity.