How to make 100 ml of 0.001 mM solution with 0.0405mM solution?

Measure 2.47 mL of the stock solution (i.e 0.0405 mM) and dilute it to the 100 mL mark with water

Explanation:

To make 100 mL of 0.001 mM solution from 0.0405mM solution, we need to determine the volume of 0.0405mM solution needed. This can be obtained as follow:

Molarity of stock (M₁) = 0.0405 mM

Volume of diluted (V₂) = 100 mL

Molarity of diluted solution (M₂) = 0.001 mM

Volume of stock solution needed (V₁) =?

M₁V₁ = M₂V₂

0.0405 × V₁ = 0.001 × 100

0.0405 × V₁ = 0.1

Divide both side by 0.0405

V₁ = 0.1 / 0.0405

V₁ = 2.47 mL

Therefore, to make 100 mL of 0.001 mM solution from 0.0405mM solution, measure 2.47 mL of the stock solution (i.e 0.0405 mM) and dilute it to the 100 mL mark with water.

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