Drink mix concentrations and absorbances for constructing a standard curve Concentration (mm) Concentration (M) Absorbance
Sample 1 200 0.200 0.52
Sample 2 77 0.077 0.18
Sample 3 32 0.032 0.08
Sample 4 300 0.300 0.77
Sample 5
a. Plot your data of absorbance versus concentration (in M) using Excel. Add a trendline and display the equation of the line to determine the slope and y intercept. Please review the video on using Excel if needed. Enter the slope of the trendline
Enter the y-intercept of the trendline
b. Using the molar absorptivity (slope) determined from your plot, calculate the expected concentration (in M) of a drink mix solution with an absorbance of 0.66.
c. Choose the statement that best answers the following question
Can you use your plot to predict the absorbance of a 214 mM solution of nickel(II) chloride and why?
A. Yes, because the same instrument is used for the measurement of the new compound.
B. Yes, because you can use the same cuvette for the measurement of the new compound
C. No, because the determined absorptivity applies only to the compound measured.