The ratio of an enzyme activity and the total amount of protein in that enzyme preparation is known as the "specific activity". This ratio intrinsically takes in account the differences in purity and activity between different enzyme preparations/purifications. For rGFP, we will define the specific activity as the relative fluorescent units per mg of total protein. Required:
a. What is the specific activity of your E3 fraction?
b. If the TA also performed the same experiment and found a lower specific activity in their E3 fraction, who (you or the TA) had the purer fraction