The gel is loaded as outlined in the LEAPS Laboratory Manual: Lane 1 – Protein Standards; Lane 2 – Induced cell lysate; Lane 3 – Column flow-through; Lane 4 – resin-bound protein; Lane 5 – purified GFP; Lane 6 – Non-induced cell lysate.
The group that created this gel were disappointed to see no band in Lane 5. To try and determine what went wrong the DNA seqeunce of the plasmid used to transfrom the E. coli used to express the proteins was determined. The sequence data revealed that there was a mutation in the linker region between coding region for GST and the coding region for GFP, and that the mutation changed the amino acid code of the fusion protein.
What would you predict that you would seeon an SDS-PAGE gel if you ran a sample of the resin AFTER the elution step? Explain you answer. (4 marks)
Answers: 1
Chemistry, 21.06.2019 13:00
Note the ph and poh values labeled with letters on the ph scale below. based on log rules and the way ph is calculated, what is the difference in [oh– ] concentration between point a and point b. a) 10^1 b) 10^5 c) 10^6 d) 10^7
Answers: 1
Chemistry, 22.06.2019 07:30
Using data from seismic waves, geologists have learned that earth’s interior is made up of several
Answers: 1
Chemistry, 22.06.2019 08:00
Define dew point. i am writing this part to be able to ask the question
Answers: 1
Chemistry, 22.06.2019 09:00
An excess of lithium oxide undergoes a synthesis reaction with water to produce lithium hydroxide li2o+h2o→2lioh if 1.05 g of water reacted, what is the theoretical yield of lithium hydroxide? a) 5.83 x 10–2 g lioh b) 1.17 x 10–1 g lioh c) 2.79 x 100 g lioh d) 1.40 x 100 g lioh
Answers: 1
The gel is loaded as outlined in the LEAPS Laboratory Manual: Lane 1 – Protein Standards; Lane 2 – I...
Social Studies, 31.10.2019 22:31
History, 31.10.2019 22:31
Chemistry, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31
Mathematics, 31.10.2019 22:31