Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIZMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH = 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5.

6. what does the symbol ah stand for? o one calorie given off by a reaction the specific heat of a substance the heat capacity of a substance the heat of reaction for a chemical reaction

Straightforward questions answered in the powerpoint slidesreaction: heating the starting materials under refluxwhat does it mean to heat under reflux? why do we choose water as the reflux solvent? what are boiling chips used for? why do we put a condenser on top of the reaction? why do we add heat and let the reaction stir for 30 minutes? why do we add sulfuric acid to the reaction after it cools as opposed to when it’s still hot? separation: filtration of precipitatewhy don’t we do an aqueous and organic extraction in the separatory funnel? why do you rinse the salicylic acid on the filter with ice cold water? purification: recrystallization of salicylic acid (no hot filtration needed)what is the difference in the amount of room temperature water vs. boiling water needed to dissolve the salicylic acid (assume a 1.2 gram yield of salicylic acid)? remember, in the lab if you need x ml of boiling water to dissolve a solid, then you should add a little more (definitely no more than 1.5 times the theoretical amount) to ensure it doesn’t recrystallize prematurely.analysis: melting point of salicylic acidwhat can you conclude if the melting point of the salicylic acid you just synthesized is 152-155oc and the 1: 1 mix of your product and “synthetic” salicylic acid is 151-154oc?

Scientific evidence tells us that the cause of earths four season is the tilt of earth as it revolves around the sun. the student is instructed to illustrate this information in a science notebook. how will the student illiterate winter in the northern hemisphere?