The isoelectric point, pI, of the protein ribonuclease T1 is 2.9 , while that of chymotrypsinogen A is 9.1 . 1. What is the net charge of ribonuclease T1 at pH 4.3 ? (POSITIVE OR NEGATIVE)
2. What is the net charge of chymotrypsinogen A at pH 8.5 ?(POSITIVE OR NEGATIVE)

The isoelectric point of threonine is 5.6 ; histidine , 7.59 .
3. During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate? (POSITIVE OR NEGATIVE)
4. During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate?(POSITIVE OR NEGATIVE)

The isoelectric point, pI, of the protein uricase is 6.3 , while that of lysozyme is 11 .
5. What is the net charge of uricase at pH 5.1 ?(POSITIVE OR NEGATIVE)
6. What is the net charge of lysozyme at pH 10.2 ? (POSITIVE OR NEGATIVE)

The isoelectric point of aspartic acid is 2.77 ; histidine , 7.59 .
7. During paper electrophoresis at pH 4.5 , toward which electrode does aspartic acid migrate? . (POSITIVE OR NEGATIVE)
8. During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate? (POSITIVE OR NEGATIVE)

The isoelectric point, pI, of the protein horse liver alcohol dehydrogenase is 6.8 , while that of chymotrypsinogen A is 9.1 .
9. What is the net charge of horse liver alcohol dehydrogenase at pH 5.1 ?(POSITIVE OR NEGATIVE)
10. What is the net charge of chymotrypsinogen A at pH 8.5 ?(POSITIVE OR NEGATIVE)
The isoelectric point of glutamine is 5.65 ; histidine , 7.59 .
11. During paper electrophoresis at pH 5.1 , toward which electrode does glutamine migrate? . (POSITIVE OR NEGATIVE)
12. During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate? (POSITIVE OR NEGATIVE)