How to find the mmol/g of ascorbic acid content in plant tissues by HPLC
So I weight out a bunch of plant tissue around 10-20mg. I homogenise them in 1000ml of HCl. I then pippet 50ul of the homogenised material into 450ul of NaPO4. Afterwards 50ul of the (plant-NaPO4) mix is injected into HPLC column. My ascorbic acid standards are (0.00001M-0.01M); 50ul of standards are also mixed with 450 ul of NaPO4; 50ul of the mix subsequently injected into HPLC
I've attached my current calculations but I dont know how to account for the dilutions when the plant are homogenised in HCL.
Thanks in advance
Answers: 1
Chemistry, 21.06.2019 12:50
what is the density of an object that has a mass of 10 g and a volumeof 5 ml? a. 0.5 g/ mlb. 2 g/mlc. 15 g/ mld. 50 g/ ml
Answers: 1
Chemistry, 22.06.2019 06:00
How many atoms of mg are present in 97.22 grams of mg? 6.022 × 1023 2.408 × 1024 4.818 × 1024 5.855 × 1025
Answers: 3
Chemistry, 22.06.2019 11:30
Compare and contrast refraction of light and sound will give brainliest
Answers: 1
Chemistry, 22.06.2019 21:30
Athe top of a hill, an athlete on a skateboard has x joules of mechanical energy. how much mechanical energy will she have at the bottom of the hill? ignore the effects of friction.
Answers: 1
How to find the mmol/g of ascorbic acid content in plant tissues by HPLC
So I weight out a bunch o...
History, 02.08.2019 19:30
Mathematics, 02.08.2019 19:30
Business, 02.08.2019 19:30
History, 02.08.2019 19:30
Mathematics, 02.08.2019 19:30
Physics, 02.08.2019 19:30
Physics, 02.08.2019 19:30
Biology, 02.08.2019 19:30
Biology, 02.08.2019 19:30
Mathematics, 02.08.2019 19:30
History, 02.08.2019 19:30
Biology, 02.08.2019 19:30