Fastqc: check if the input fastq file(s) are sanger/illumina 1.9 encoding fastq groomer: reformat fastq file(s) to sanger/illumina 1.9 encoding if necessary fastq trimmer or trimmomatic: remove low quality regions alignment (e. g. bowtie2/bwa/hisat): align reads to a reference genome, giving bam/sam files sam-to-bam: convert the output file(s) to bam files if the output from the last step is a sam file variant calling (e. g. freebayes): call variants using output bam file and reference genome, giving vcf file

We as humans write math expression in infix notation, e.g. 5 + 2 (the operators are written in-between the operands). in a computer’s language, however, it is preferred to have the operators on the right side of the operands, i.e. 5 2 +. for more complex expressions that include parenthesis and multiple operators, a compiler has to convert the expression into postfix first and then evaluate the resulting postfix.write a program that takes an “infix” expression as input, uses stacks to convert it into postfix expression, and finally evaluates it. it must support the following operations: + - / * ^ % (example infix expression: (7 - 3) / (2 + 2)postfix expression: 7 3 - 2 2 + /result: 1guidelines: 1. you will need to use stacks in three placesa. one for the parenthesis check [char stack]b. one during infix to postfix [char stack]c. one during evaluation [int stack]for a and b above, you can use same array and same push, pop method as both ofthem are char. but for evaluation you have int stack and you might consider to createanother push pop method to handle it. maybe push_int, pop_int, etc. or find otherstrategy to utilize existing push pop method2. you can create a function for obtaining operator priority. that function should take anoperator as input and return its priority as an integer. this function will you a lot andreduce repeated code3. during evaluation you will need to convert char into integer. example for single digit: char c = '5'; int x = c - '0';

Researchers measured the data speeds for a particular smartphone carrier at 50 airports. the highest speed measured was 78.1 mbps. the complete list of 50 data speeds has a mean of x overbarequals16.11 mbps and a standard deviation of sequals18.65 mbps. a. what is the difference between carrier's highest data speed and the mean of all 50 data speeds? b. how many standard deviations is that [the difference found in part (a)]? c. convert the carrier's highest data speed to a z score. d. if we consider data speeds that convert to z scores between minus2 and 2 to be neither significantly low nor significantly high, is the carrier's highest data speed significant? a. the difference is nothing mbps.

Many everyday occurrences can be represented as a binary bit. for example, a door is open or closed, the stove is on or off, and the fog is asleep or awake. could relationships be represented as a binary value? give example.