8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Air is to be cooled in the evaporator section of a refrigerator by passing it over a bank of 0.8-cm-outer-diameter and 0.4-m-long tubes inside which the refrigerant is evaporating at -20°c. air approaches the tube bank in the normal direction at 0°c and 1 atm with a mean velocity of 4 m/s. the tubes are arranged in-line with longitudinal and transverse pitches of sl- st 1.5 cm. there are 30 rows in the flow direction with 15 tubes in each row. determine (a) the refrigeration capacity of this system and (b) pressure drop across the tube bank. evaluate the air properties at an assumed mean temperature of -5°c and 1 atm. is this a good assumption?

Acertain flow of air (at stp) has a velocity distribution given by v i (in ft/s). if this flow is going through a 4 ft square area in the yz-plane (centered at the origin), what is the mass flow rate (in lbm/s)?

Refrigerant-134a enters the compressor of a refrigerator as superheated vapor at 0.14 mpa and -10°c at a rate of 0.05 ka/s and leaves at 0.8 mpa and 50°c. the refrigerant is cooied in the condenser to 0.72 mpa and 26'c. it is then throttled to 0.15 mpa. sketch the t-s diagram for the system and evaluate: 6) the rate of heat removai from the refrigerated space (kw), it) the power input to the compressor (kw), ii) the isentropic efficiency of the compressor (%), and iv) the cop of the refrigerator.