Engineering, 11.04.2020 04:02 lilroach4
A dual-wavelength spectrometer uses 780 nm and 830 nm. The molar extinction coefficients for oxy- hemoglobin (HbO2) and deoxy-hemoglobin (Hb) at these two wavelengths are: e_HbO2_780nm = 710 cm 1M-1, e_Hb_780nm = 1075 cm-1M-1, e_HbO2_830nm = 974 cm-1M-1, e_Hb_830nm = 693 cm-1M-1. The spectrometer measures absorption coefficients at these two wavelengths are: Ua_780nm = 0.057 cm-", Ua_830nm = 0.063 cm-, please calculate the concentration for HbO2 and Hb (in unit of mM).
a. [HbO2] = 18.45 uM; [Hb] = 12.11 UM
b. [HbO2] = 8.45 UM; [Hb] = 22.11 UM
c. [Hb] = 8.45 uM; [HbO2] = 22.11 UM
d. [Hb] = 19.44 UM; [HbO2] = 50.85 UM
Answers: 1
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A dual-wavelength spectrometer uses 780 nm and 830 nm. The molar extinction coefficients for oxy- he...
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