The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations, and circle final answers):
[S] mM No Inhibitor V0 (mmol/min) Inhibitor A V1 (mmol/min) Inhibitor B V2 (mmol/min)
3 3.90 2.40 1.29
6 6.00 3.60 2.40
12 8.40 5.10 3.90
24 10.8 6.60 6.30
36 12.0 7.20 8.55
a. Write out the Michaelis-Menten and Lineweaver-Burke equations
b. Plot all of the data on one plot as Michaelis-Menten saturation curves
c. Plot all of the data on one plot in the Lineweaver-Burk format
d. Define Km and Vmax