Initially, Elise was concerned that the causative agent could have been Staphylococcus aureus, a Gram-positive bacterium, or P. aeruginosa, a Gram-negative bacterium; both are common culprits in piercing infections. What culture methods would allow for isolation and differentiation of these two bacteria

Gram staining method. MacConkay agar.

Explanation: Gram staining is a differential media. It is used to differentiate between gram positive and gram negative organisms.

Pseudomonas aeruginosa can be isolated from samples via an enrichment procedure. It is confirmed through use of selective and differential media

Staphylococci are catalase positive organisms. Catalase-positive cultures bubble immediately.

Gram staining method

Explanation:

One great differential method known is Gram staining. It uses certain dyes for the making of bacterial cells to standout over its background. The gram staining method is used in the differentiation of gram positive and gram negative organisms.