What issue started the violence in the Kansas territory?
the fight that broke out in the Senate over slavery
the Senate's refusal to admit Kansas as a slave state
the vote over whether to allow slavery
the start of the abolitionist movement

the fight that broke out in the Senate over slavery

Explanation:

That caused the conflict between pro-slavery and anti-slavery settlers. The Act made it possible for the Kansas and Nebraska territories to open to slavery. This Act eliminated the Missouri Compromise line and Northerners were angry about that.

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a.that is my answer.

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