The 1854 Ostend Manifesto a. prohibited enslaved labor in the Hawaiian Islands. b. was directed at limiting England's influence in the Caribbean. c. enraged southern enslavers. d. was part of an attempt by the United States to acquire Cuba. e. saw several European powers denounce the American enslaved labor system.

Correct

d. was part of an attempt by the United States to acquire Cuba.

Explanation:

The 1854 Ostend Manifesto a document written in 1854 that described the rationale for the United States to purchase Cuba from Spain. And, also, the document was implying that, should Spain refuse US request, they will go to war over the island.

Cuba's annexation had long been a goal of U.S. Southern States slaveholding expansionists due to their ambition of setting up a new territory for their slaves and slave business.

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four sitting presidents have been assassinated while in office but the first was abraham lincoln,