All of the following established settlements in North America except for

The Portuguese Did not establish colonies during the colonial era

Explanation:

The Portuguese explorers and settlers, in the beginning, settle in the North American parts of Labrador, Newfoundland, and Nova Scotia claimed lands as a part of the Portuguese Empire. The main occupation of this region was cod fisheries, later abandoned. The Portuguese focused on the exploration of South America where they found resources like Brazilwood and later on gold and Silver. The economy of Portuguese flourished with introducing of the sugar plantations in Brazil colonies.  

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answer:   migrations were important in the neolithic period. "two major migrations passing through southeastern europe were confirmed by the means of archaeo-genetic studies."

the first migration was the early neolithic period -- 6,000 before common era (bce) -- when the first farmers, from anatolia -- asia minor -- spread through europe. the second migration occurred during the early bronze age (3,000-2,500 bce) when the so-called "steppe population," from the eurasian steppe, replaced much of northern europe's previous population.

the first farmers of northern and western europe passed through southeastern europe with limited hunter-gatherer genetic admixture, which occurs when two or more previously isolated populations begin interbreeding. however, some groups that remained mixed extensively -- without the male-biased, hunter-gatherer admixture that prevailed later in the north and west, according to latest discoveries. southeastern europe continued to be a nexus between east and west, with intermittent genetic contact with the steppe people up to 2,000 years before the migrations that replaced much of northern europe's population.

"in some places, hunter-gatherers and incoming farmers seem to have mixed very quickly," says iain mathieson, a geneticist at the university of pennsylvania, who was first author of the paper. "but, mostly, the two groups remained isolated, at least for the first few hundred years. these hunter-gatherers had been living there for thousands of years, and it must have been quite a shock to have these new people show up -- with a completely different lifestyle and appearance."