What was the Outcome/Significance of Charles I taking the throne and mistreating Parliament

It led to the Civil war in England that ended with Charles death and creation of Republic. Charles' religious policy alienated most of the people against him and that was more deepened by him not allowing Parliament to participate in the decision making process.

Explanation:

In 1642 a civil war started between supporters of Charles Stuart (Royalists) and supporters of Parliament. After defeat at the battle of Naseby, Charles was dethroned in 1649. Republic, under the reign of Oliver Cromwell was established.

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