By closing japanese harbors to most foreigners in
the 160os, the tokugawa shogunate attempted to
(1) protect japan from european influence
(2) increase japanese agricultural production
(3) eliminate japan’s influence on southeast asia
(4) destroy traditional japanese culture

By closing Japanese harbors to most foreigners in the 1600's the Tokugawa shogunate attempted to (1) protect Japan from European influence. At the time of the Tokugawa shogunate, there was a high sentiment of nationalism and isolationism. Having been isolated not only geographically wise, Japan wanted to isolate itself from foreigners who it found greedy and outside of their social hierarchy and wanted to retain its culture. 

The middle east is where the domestication of animals began