In the plaque assay for bacteriophages, A. only temperate phages may be used. B. coculture with bacteria is not required. C. the plaque is a clear zone caused by a zone of lysis. D. the plaque is an opaque area caused by a high concentration of virus.

C

Explanation:

Phage plaques. Plaques are clear zones formed in a lawn of cells due to lysis by phage. ... A diversity of plaque sizes can result if the phage infect cells at different times during the bacterial growth phase: phage that adsorb early make larger plaques than those that adsorb later

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