You are interested in epithelial tissue formation and work in a lab studying Madin-Darby canine kidney (MDCK) cells. MDCK cells spontaneously form hollow epithelial vesicles. Staining these vesicles with an anti-actin antibody demonstrates the existence of apical microvilli near the central cavity of the vesicle. A new postdoctoral fellow in the lab comes to you in a panic. You had given her some dissociated MDCK cells that have been cultured as single cells in a collagen matrix. Because the cells did not look obviously polarized to her, she stained the dissociated MDCK cells to visualize the microvilli and she does not see any signs of polarized microvilli localization in these cells. Explain why you are confident that you did not give her the wrong cell line.

Name the bone/structure/process that fits the following description: a) a bone with a complex shape. b) found in the medullary cavity. c) a large, rough process. d) it is thin, like a plate. e) shaft of a long bone. f) a mature bone cell. g) a large, round, smooth process. h) it covers outer surface of bone. i) a shallow depression on surface of bone. j) a roughly cube-shaped bone. k) the covering of epiphysis. l) a very narrow ridge of bone. m) space between lamellae for an osteocyte. n) chamber within diaphysis o) also called wormian bone. p) it secretes new matrix. q) end of a long bone. r) a small, round hole. s) a matrix, arrangement in spongy bone. t) it is a chamber within a bone. u) a network allowing osteocytes to receive nutrients. v) one example is the patella. w) it houses artery, vein and nerve. x) almost flat, smooth surface of a bone. y) a narrow, pointed process.