Which processes would be most directly affected if a thylakoid membrane is punctured so that the interior of the thylakoid is no longer separated from the stroma?

there is no correct list among the choices you've provided.

the list might look something like this:

-- baby human

-- piccolo

-- flute

-- soprano singer

-- trumpet

-- tenor singer

-- trombone

-- baritone singer

-- baritone horn

-- bass singer

-- tuba

since i had to make up the list myself, i made it easy on myself, and i didn't try to shuffle the string instruments and woodwinds into it too.