The molecular weight of an unspecified protein, at physiological conditions, is 70,000 dalton as determined by gel filtration chromatography. the sds-polyacrylamide gel electrophoresis (sds page) of the protein yields a single band corresponding to molecular weight of 70,000 dalton. however, in the presence of the reducing agent, β-mercaptoethanol, the sds page shows two bands, corresponding to molecular weights of 30,000 and 20,000 dalton.

1: from these data, describe the native protein in terms of the number of subunits present, their molecular weight, stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between the subunits.

2: how would your answers to problem (a) change if the molecular weight of the protein was 100,000 dalton?

3: you synthesized two 200-aminoacid long polypeptides, one being poly-glutamic acid and another one poly-lysine. you accidentally mixed the peptides and need to separate them. suggest schematics for their separation. can you use iec? gec?

Do you think the gene eef1 alpha1 supports cell theory? explain your response.

He did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis

Which of the following is a testable hypotheses?