To clone the entire coding region of p53 into the plasmid, which restriction enzyme(s) could you use to cut both your pcr product and the pcdna3 plasmid? select all that apply. select all that apply. e (ecori) b (bamhi) h (hindiii) s (smai) x (xbai) n (noti)

E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI).

Explanation:

Only SmaI does not have a recognition site in the polylinker of pCDNA vector (See map attached). So, in theory, any of the other enzymes:  E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI) could be used. It would be of importance to see in silico the digestion of regions flanking the p53 gene.

its raceist

explanation:

it dosn't have red,yellow,green,blue,indigo,violet,or purple

image result for how many calories of energy will 20 grams of carbohydrates provide in one gram provides 4 calories

consuming the right balance of protein, carbohydrates and fat can you maintain a healthy weight and optimize your energy levels. protein and carbohydrates both contain 4 calories per gram, while fat provides 9 calories per gram.