An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?

Innoculation of cell tissues

Explanation: Inocculation is the growing of microbes in a nutrient based media. There are different ways of innoculating we have broths or Agar plates.

Media should be culture in an aseptic environment under a sterile condition to avoid contamination.

Bacteria, virus can be innoculated in a media.

The requirement for growth depends on the cell to be cultured. It includes,

amino acids

carbohydrates

vitamins

minerals

growth factors

hormones

CO2 or O2

pH buffer to maintain relatively neutral pH

osmotic pressure

temperature (typically 37 degrees C

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.