When the F1 portion of the ATP synthetase complex is removed from the mitochondrial membrane and studied in solution, it functions as an ATPase. Why does it not function as an ATP synthetase

How is it regenerated? When the F1 portion of the ATP synthetase complex is removed from the mitochondrial membrane and studied in solution, it functions as an ATPase. Why does it not function as an ATP synthetase? The standard free-energy change for ATP hydrolysis is -30.5 kJ/mol.

what school and grade?

b) the inside picture would look at a very high temperature in a very low ph