You wish to express a particular peptide in E. coli using a high copy number plasmid. You have the amino acid sequence for the peptide. a. Explain the experimental process for generating and selecting the genetically engineered E coli using restriction enzymes, ligase, E. coli, plasmid with neomycin resistance, and known amino acid sequence. b. What control elements would you place on the plasmid to regulate expression and to prevent read-through

What are some things that limit population growth?

Asea turtle has washed up on a remote section of a beach. this known as a stranding occurs when a dead,sick or injured sea turtle washed up on the shoreline. which statment best explain why stranding should be reported immediately to local authorities

Refer to this portion of a dichotomous key for fish identification to answer the question. 1. (a) has a single dorsal fin ® 5 (b) has a double dorsal fin ® 2 2. (a) one fin is spiny, the other is smooth ® 3 (b) one fin is not spiny or smooth ® 4 5. (a) has small fin on back near tail ® 6 (b) has no fin on back near tail ® 7 6. (a) has barbs near the mouth ® catfish (b) does not have barbs near the mouth ® 10 7. (a) tail is asymmetrical ® 8 (b) tail is symmetrical ® 9 10. (a) scales are small ® trout (b) scales are large ® whitefish what is the next step to complete to identify a fish that has a single dorsal fin, no fin on the back near the tail, and no barbs near the mouth? step 2 step 5 step 7 step 10