Using established recombinant DNA technology, you insert a gene from a human immune cell into a bacterium. The bacterium expresses the protein corresponding to the inserted DNA. To your disappointment, you discover that the protein produced is useless and is found to contain many more amino acids than does the protein made by eukaryotic cells. Assuming that there is no mutation in the human gene, what is a reasonable explanation for why this happened

The group of choices associated with the question is -

The inserted gene does not have a start codon.

The inserted gene does not have a stop codon.

The inserted gene is not present in bacteria.

The inserted gene contained introns.

None of the above.

The correct answer is - The inserted gene contained introns.

Explanation:

In the given case says that one gene inserted from human to bacteria that produces a protein with more amino acids than humans. In eukaryotic organisms, there is a process that removes the unwanted mRNA part during transcription and joins exons together, called splicing to form functional mRNA.

In this case. splicing does not take place as bacteria is a prokaryotic organism and splicing is exclusive to eukaryotic organisms like humans.

So introns are also translated into protein in bacteria due to lack of splicing genes.

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