How could you test to see if an enzyme was completely depleted during an experiment?

              Enzymes do NOT get depleted during a reaction. Only the substrate and/or cofactors will get used up. The enzyme remains unchanged

the bald eagle got 50 because everything gets 10% of what they consume and since 50 is ten % of 500 hundred that means the   grasshopper had to have consumed a plant with 5,000 calories of energy