The kind of biome that develops in an area is determined by temperature and

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The kind of biome that develops in an area is determined by temperature and --

The kind of biome that develops in an area is determined by temperature and precipitation. These are the abiotic factors that help determine the environment of the biome. They tell whether a biome is humid, dry freezy or hot. 

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Plants, sunlight, growth and artificial sunlight

The answer is joseph is observing the colour of the reaction mixture to see whether proteins are present in the given solution.