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Imagine that twenty i.u.’s of enzyme z were catalyzing the above reaction for one minute, under vmaxconditions, in a 3.00 ml assay volume. the assay is buffered with 20 mm phosphate buffer, ph 7.60. what will the ph be at the end of that one minute?

Achemistry student needs to standardize a fresh solution of sodium hydroxide. he carefully weighs out of oxalic acid , a diprotic acid that can be purchased inexpensively in high purity, and dissolves it in of distilled water. the student then titrates the oxalic acid solution with his sodium hydroxide solution. when the titration reaches the equivalence point, the student finds he has used of sodium hydroxide solution.calculate the molarity of the student's sodium hydroxide solution. be sure your answer has the correct number of significant digits.

Answer asap need by wednesday morning explain how a buffer works, using an ethanoic acid/sodium ethanoate system including how the system resists changes in ph upon addition of a small amount of base and upon addition of a small amount of acid respectively. include the following calculations in your i. calculate the ph of a solution made by mixing 25cm3 0.1m ch3cooh and 40cm3 0.1m ch3coo-na+. [ka = 1.74 x 10-5 m] ii. calculate the ph following the addition of a 10cm3 portion of 0.08 m naoh to 500cm3 of this buffer solution. iii. calculate the ph following the addition of a 10cm3 portion of 0.08 m hcl to 200cm3 of the original buffer solution.