What technique allows an investigation of the microheterogeneity of a membrane so that one can see localized differences in different parts of the membrane?

Freeze Fracture replication

Explanation:

Freeze Fracture replication is a technique that allows investigation of the microheterogeneity of a membrane so that one can see localized differences in different parts of the membrane.

Freeze fracture replication is a technique used with electron microscopy to splits frozen cell membranes apart for visualization of the membrane structure and the organization of proteins within the sea of phospholipids.

The four steps in making a freeze-fracture replication are

(i) rapid freezing,

(ii) fracturing,

(iii) replication and

(iv) replica cleaning.

think its the third option