What is the next ATP gain at this stage of cellular respiration?

The energy to split glucose is provided by two molecules of ATP. ... As a result, there is a net gain of two ATP molecules during glycolysis. During this stage, high-energy electrons are also transferred to molecules of NAD+ to produce two molecules of NADH, another energy-carrying molecule.

the relative atomic mass of an element is the mass of one atom of that element, relative to 1/12th of the mass of a  1212c atom. to convert to an absolute mass in kg, it is necessary to multiply by  1.661×10−271.661×10−27.

the relative atomic mass is roughly equal to the number of neutrons, plus the number of protons, in the nucleus of the atom.

for elements with more than one stable isotope (different types of nucleus with different numbers of neutrons but the same number of protons), the quoted mass is usually the average mass, weighted by the relative abundance of the different isotopes.